In season 1 episode "Witch Hunt" they go to the prom as a couple, holding hands as they enter and afterwards dance together.Annaliese, however, is more open about who they are to each other.Del Martin is a feminist Lesbian and activist, and co-founder of Daughters of Bilitis.
Izzy is a Psychology Graduate student who is trying to make enough money to pay for school so she decides to take her friends advice and becomes an escort.
Assente confirms his homosexuality in private conversation with Pope Pius XIII in episode 2 of season 1.
Sanchez is accused of "sexual disturbances", which seems to be the euphemism for homosexual tendencies.
Michel is from the original Gilmore Girls series and is married to Frederick (David Greenman), though he is not seen.
Donald is a newer Stars Hollow resident introduced upon discussion of the first potential LGBT Pride festival for the town, where some more notable characters' sexualities are mentioned and/or danced around.